This article explores the integration and validation of the Absorbance 96 Automate with the Eppendorf epMotion® liquid handling system for automating cell-based assays.
Sponsored Content by ByonoyMar 18 2024Reviewed by Maria Osipova Automating microplate reading on liquid handling systems has brought about a substantial advancement in laboratory system workflows, seamlessly combining the precise LHS capabilities of liquid handlers with the analytical readout efficacy of the microplate reader.This integrated approach, allowing for the simultaneous measurements of multiple samples, enhances throughput, allowing researchers to conduct their work more efficiently.
Results demonstrate comparable reproducibility and optical density readouts between the integrated solution and its benchtop counterpart, validating the robustness and reliability of Absorbance 96 Automate for automated cell-based assays. Samples were automatically subjected to absorbance measurement at 490 nm using the Absorbance 96 Automate positioned on the deck of the epMotion® workstation.
Figure 1. HeLa cells were treated with Staurosporine to induce cellular cytotoxicity. Assessment of cellular cytotoxicity was conducted using the CytoTox 96® Non-radioactive Cytotoxicity Assay kit. Absorbance measurements at 490 nm were performed using both the on-deck integrated Absorbance 96 Automate on the epMotion® liquid handling system and the benchtop microplate reader, GloMax® Discover.
Summary The integration of the Absorbance 96 Automate with the epMotion® deck is characterized by its seamless nature. It offers a convenient automatic workflow for evaluating cell-based assays with precision and enabling high-throughput capabilities. About Byonoy The Byonoy GmbH was founded in 2015 as a spin-off from the University of Kiel and is situated in Hamburg. Our core competence is to develop innovative microplate readers based on photometric measurements for laboratory use.
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