This article presents a study on integrating the Absorbance 96 Automate with Eppendorf's epMotion® system.
Sponsored Content by ByonoyMar 18 2024Reviewed by Maria Osipova To understand the bacterial growth kinetics and to optimize biomass and metabolite production, it is vital to monitor the growth of bacteria in culture.1 The predominant technique for quantifying bacterial growth encompasses the measurement of optical density at 600 nm using either a microplate reader or photometer.Using microplate readers for such a measurement will provide increased throughput and improved temporal resolution.
The results reveal comparable readouts, identical reproducibility, and performance sensitivity, which affirm the efficiency of the Absorbance 96 Automate in conducting throughput OD600 measurements. Results Comparative analysis was conducted with the GloMax® Discover microplate reader from Promega. Bacterial samples with varying dilution factors were measured by recording absorbance values either at 595 nm or at 600 nm . Similar OD values and a significant correlation were observed between both instruments; see Figure 1.
Further, the comparative analysis assessed bacterial growth measurements using both manual and automated workflows employing the epMotion® and Absorbance 96 Automate workflow solution, outcomes of which revealed a significant correlation between the two methodologies , indicating similarly efficient performances.
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