Researchers discover potential new prescription strategy for ischemic stroke lsu
Brain samples of the anterior and posterior ipsilesional cortex and subcortex regions were harvested 24 h after reperfusion, flash-frozen in liquid nitrogen, and stored at − 80 °C until use. Tissue samples from each region were homogenized using TRIzol® reagent according to the manufacturer’s protocol. Total RNA was prepared using RNeasy columns . RNA quantity and purity were assessed using the NanoDrop One spectrophotometer .
After priming, the chip was loaded with samples, and the primer reaction mixes within 1 h to reduce the pressure loss within the chip. Thus, 5 µL of each primer reaction mix and each sample were loaded into respective inlets. Samples and primer reaction mixes were loaded into the chip into the Juno and running the Load mix 96.96 GE script.
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