This article outlines the in vitro efficacy evaluation methodologies employed by Sino Biological for antibody drugs.
Sponsored Content by Sino Biological Inc.Mar 14 2024Reviewed by Maria Osipova Antibody drugs such as monoclonal antibodies , bispecific antibodies, antibody-drug conjugates , and nanobodies garnered increased interest in research and development circles.
Integrated in vitro efficacy evaluation services CDC assay service Complement-dependent cytotoxicity assay service Complement-dependent cytotoxicity in mAb-based therapy starts with binding the complement protein C1q to the Fc domain of mAbs for the opsonization of a target cell. In the process of pathogen infection, instead of stopping the virus from entering cells, the original neutralizing antibodies boost virus replication in the body and bring about significant pathological reactions by interacting with Fc receptors or complements to infect macrophages and granulocytes.
Once the antibody has bound to the target antigen on the cell surface of tumor cells, the ADC drug is endocytosed by the tumor cells and encapsulated within the intracellular lysosomes to release cytotoxic molecules that impair DNA or prevent the division of tumor cells, consequently killing the tumor cells.
Sino Biological can supply pseudovirus assays, immune checkpoints, and cytokine neutralizing/blocking antibody functional validation services, which includes both cell activity assay and neutralization titer assay. Sino Biological has created high-quality proteins for use across several research areas, including Fc receptor, immune checkpoint, and biotinylated proteins.
7-AAD is a nucleic acid dye that does not have the capacity to penetrate cell membranes, but 7-AAD can permeate the cell membranes of cells in the mid- to late-stages of apoptosis, or even infiltrate dead cells and stain the nucleus red. The analysis of mitochondrial membrane potential through JC-1 assays. Image Credit: Sino Biological Inc.
Three commonplace MLR assays include DC+T, PBMC+PBMC, and T+T. Among them, DC+T is a classic assay frequently used to assess the enhancement effect of antibody drugs on T-cell response. Immobilized Human PD-1 Protein at 2 μg/mL can bind Human PD-L2 Protein , the EC50 is 45-280 ng/mL. Image Credit: Sino Biological Inc.Using the Octet RED System, the affinity constant of Human PD-1 Protein , bound to Atezolizumab was 0.3 nM. Image Credit: Sino Biological Inc.Control antibody production service Sino Biological offers research-grade biosimilar control antibody production services of the highest standard.
FACS antibodies Sino Biological can provide a range of high-grade FACS antibodies . Due to the high specificity, affinity, and exceptional signal-to-noise ratio, they can be employed in MLR assays to determine the levels of various T lymphocyte subpopulations or DC cells. Flow cytometric analysis of Human CD3 expression on human peripheral blood lymphocytes. Image Credit: Sino Biological Inc.Flow cytometric analysis of Human CD4 expression on human peripheral blood lymphocytes.
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