Nature research paper: HIV silencing and cell survival signatures in infected T cell reservoirs
. To generate the final 1× reaction mixture concentration, beads were soaked in 2× PCR master mix on a shaker for 30 min in the dark. Next, the beads were centrifuged and loaded into a 3 ml syringe. The remaining 1× PCR master mix was loaded into a separate 3 ml syringe. Finally, the beads and 1× PCR master mix were reinjected in the microfluidic device to encapsulate the beads into 70 µm droplets.
samples that differed qualitatively from other replicates by having lower exonic reads or higher rRNA content were removed. If all HIV-DNAsamples were removed for a participant, that participant was excluded from further analysis. After the removal of 31 FIND-seq-sorted samples in this curation process, 22 HIV-DNA
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