Nature research paper: Integrated intracellular organization and its variations in human iPS cells
. The limits of the cell boundary segmentation algorithm include potential errors for the very top slices of each cell. Desmosomes, which localize to the cell periphery at the top of the cell, demonstrate this caveat well. Four sequential z-slices moving upwards towards the top of the cell-membrane dye signal are shown.
This segmentation works well for nuclei in most of interphase ). However, SMC-1A moves from the cytoplasm back into the nucleus after mitosis. The amount of tagged SMC-1A protein in the nucleus and thus its segmentation depends on how far into interphase a cell is. Three examples of tagged SMC-1A are shown along with the target segmentations . For a cell in early interphase SMC-1A is both in the cytoplasm and nucleus, but at low levels such that the target segmentation is quite sparse.
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