Tend to get sick when the air is dry? New research helps explain why PNASNexus
$$\begin{eqnarray} k=\left\,\ln \left. \end{eqnarray}$$Protein concentrations for the aerosolization solution in each individual chamber experiment were measured in a Qubit 4 fluorometer with Qubit Protein Assay Kit .A Micro-Orifice Uniform Deposition Impactor was used for size-fractionated particle characterization in separate chamber runs where humidity was controlled at 25% and 60% RH.Heavy-duty aluminum foil was punched into 47-mm-diameter circles.
Six gelatin filters with 47 mm diameter were placed with sterile tweezers in a 50 mL centrifuge conical tube containing 12 mL DMEM. The mixture was warmed and mixed at 37°C until completely dissolved; 5 mL of this gelatin-DMEM mixture was evenly distributed on sterile aluminum surface plates fitted for each of the MOUDI stages described above.
Volumes of 8 mL MHV in artificial saliva was nebulized for 30 seconds at 14 kPA as previously described. In separate trials, the aerosolized virus was allowed to age for 20 minutes at the RH extremes circumscribing these experiments .Eleven 50 mL sterile centrifuge tubes with 10 mL DMEM were placed in a water bath at 37°C to warm the solution.
The solubilized gelatin was subsequently vortexed for 10 seconds, and 100 µL were transferred to 1.5 mL microcentrifuge tubes containing 100µL genomic preservative . RNA was extracted following the Quick-RNA Viral Kit protocol, and was eluted into 15 μL molecular biology grade water and processed for RT-qPCR, as previously described. The RT-qPCR recovery from each MOUDI stage was compared to its counterparts at the RH extremes circumscribing this study .
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