Two unions are taking industrial action on Wednesday in a dispute over pay and conditions.
A fresh round of strikes by ambulance staff could create "significant disruption", health bosses say.
Nick Smith, director of operations at YAS, said plans were in place to "mitigate the risks" but asked people to use the 999 number responsibly.Mr Smith said he expected Wednesday's action to have a greater impact than a previous strike on 21 December, as it involves all ambulance employees - not just 999 response crews.
Rachel Harrison, GMB National Secretary, said the industrial action was in response to the "NHS's crushing recruitment and retention crisis" and the government's 4% pay offer which was a "massive real terms pay cut". "It also allowed time for the Government to talk to us about pay, but Ministers have dithered and postured, wasting valuable time.
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