Whole-body cellular mapping in mouse using standard IgG antibodies
). By facilitating the use of off-the-shelf IgG antibodies, wildDISCO broadens the applicability of whole-body clearing and imaging techniques. Our presentation of whole mouse body atlases will enable a deeper understanding of complex biological systems.
To determine the efficiency of methylene blue staining after incubation with different cyclodextrin buffers, samples were cut in half along the middle line to evaluate the efficacy of the inner tissue staining. The methylene blue-stained mouse brains were cryosectioned into 10 μm slices for higher resolution images using Zeiss Axio Imager.M2 microscopy. The images of the samples were analyzed by ImageJ for profile plot along and the pixels were quantified under threshold gray value.
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