This updated reference now includes a study indicating that thrombin time results using capillary blood samples are comparable to those using venous blood samples.
Centrifugation: 2000-2500 g for 15 minutes or similar regimen to produce platelet-poor plasma Storage: Up to 8 hours at room temperature ; plasma sample should be frozen within 1-2 hours; specimen is stable for one month at -20°C, or 6-9 months at -80°CThrombin time is a screening coagulation test designed to assess fibrin formation from fibrinogen in plasma.
Thrombin time is performed as the next step in the evaluation of abnormally prolonged activated partial thromboplastin time or prothrombin time .The thrombin time is used to assess unexplained prolongation of PT or PTT .The thrombin time is very sensitive to unfractionated heparin and might be used for detection of accidental heparin contamination of a plasma specimen. Low albumin levels in the plasma sample can cause thrombin time prolongation due to delayed fibrin polymerization.A study by Fliervoet et al indicated that thrombin time results using capillary blood samples are comparable to those using venous blood samples; the same held true for the capillary/venous results for the international normalized ratio , PT, fibrinogen, and D-dimer. The investigators determined the mean relative difference in thrombin time between capillary and venous samples to be -1.7%.
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