Researchers make significant step in fight against drugresistance in TB NatureComms
reads of the outer wells were averaged to provide a background value that was removed from the raw ODvalues of columns 2–10 were represented as relative to the solvent only column . The MIC was determined using a nonlinear fitting of data to the Gompertz equationCRISPRi MICs were performed as above with the following variations. Knockdown strains were pre-depleted for 5 days in the presence of 300 ng/mL anhydrotetracycline and 20 µg/mL kanamycin prior to inoculation into MIC assays.
Fold changes in susceptibility of each resistant mutant against each compound were determined relative to the parental strains used in each replicate experiment. For each resistant strain, all MICs and fold changes in susceptibility relative to the parental strain were determined from at least four biological replicates. Collateral susceptibility was defined by an average threefold change in MIC across replicate experiments.
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