When editing bacteria with CRISPR, less is more NatureComms
; pDC786, pDC841, pDC876, pDC869, pDC829, pDC860, pDC889, pDC862, and pDC886) and selected for on appropriate antibiotics. Individual colonies were inoculated into LB with appropriate antibiotics and incubated at 37 °C shaking at 220 rpm overnight. Cultures were then back-diluted into 15 mL of fresh LB with antibiotics and grown to an ABSof 0.6–0.8. 5 mL of this culture was mixed with 1 mL of STOP mix, 95% of 100% ethanol and 5% hot phenol .
The samples were then spun down at 13,000 rpm at 12 °C for 15 min. The upper phase was then transferred into a sterile 2 mL Eppendorf tube where 1.4 mL of 30:1 ethanol: sodium acetate pH 6.5 was added. The RNA was precipitated at −20 °C for 2 h. The samples were then spun down at 13,000 rpm at 4 °C for 30 minutes. The supernatant was removed and 500 µL of 70% ethanol was added to the pellet. The samples were spun down at 13,000 rpm at 4 °C for 10 min.
United States Latest News, United States Headlines
Similar News:You can also read news stories similar to this one that we have collected from other news sources.
Equatorial waves as useful precursors to tropical cyclone occurrence and intensification - Nature CommunicationsHere, the authors show that pre-existing equatorial waves are useful precursors to tropical cyclone occurrence and intensification up to two weeks ahead.
Read more »
Practical wisdom and virtue ethics for knowledge co-production in sustainability science - Nature SustainabilityPhilosophers in the Western tradition of virtue ethics have long considered practical wisdom a central virtue. This Perspective suggests that virtue ethics and practical wisdom can enrich the work of sustainability researchers, helping them to navigate the challenges of co-producing knowledge and effecting transformative change.
Read more »
Dearomative triple elementalization of quinolines driven by visible light - Nature CommunicationsOrganoboron and organosilicon compounds have potential applications in various fields including materials science and medicinal chemistry. Here the authors report a protocol that enables the dearomative carbo-sila-boration of quinolines.
Read more »
Net loss of biomass predicted for tropical biomes in a changing climate - Nature Climate ChangePrecipitation and temperature affect biomass and carbon storage in the tropics. This study shows that warming-driven contraction of humid regions and expansion of areas with dry periods could double carbon losses, with about one-third associated with decline of humid areas in South America.
Read more »
2022 – toughing out the trough - Nature BiotechnologyInvestor faith in biotech stocks fell further in 2022 after a flat performance for the sector in the previous year, according to our latest Data Page. Biotech IPOs raised less than one-eighth of the money they did in 2021
Read more »
Universal logic with encoded spin qubits in silicon - NatureNature research paper: Universal logic with encoded spin qubits in silicon
Read more »