Nature research paper: Synonymous mutations in representative yeast genes are mostly strongly non-neutral
population per mutant library assessed by flow cytometry.
Green fluorescence was analyzed using the BL2 detector that measured the output from the 488-nm laser . In control flow cytometry profiles, the two peaks respectively represent cells in the G1 and G2/M cell-cycle stages , Fractions of synonymous and nonsynonymous mutants among designed but unobserved mutants and those among observed mutants. Nonsense mutants are not considered. Numbers in the bars are numbers of mutants.
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