Researchers uncover new potential for botulinum neurotoxin E in therapeutic and cosmetic applications NatureComms
. All mutations were generated by two-step PCR and verified by DNA sequencing. BoNT/E and mutants carrying C-terminal His6-tag were purified on Co-Talon matrix and eluted with 50 mM Tris–HCl , 150 mM NaCl, and 250 mM imidazole. For proteolytic activation and removal of an affinity tag, BoNT/E was incubated for 16 h at room temperature with 0.01 U bovine thrombin per µg of BoNT. Subsequent gel filtration was performed in phosphate-buffered saline .
For the HE–G6 complex, the best crystals were obtained in a reservoir containing 0.1 M HEPES, pH 7.0, 0.2 M NaCl, and 18% PEG 3350, and the crystals were cryoprotected in the mother liquor supplemented with 20% ethylene glycol. The best crystals for the HE–SV2Ac–G6 complex were obtained in a reservoir containing 0.1 M HEPES, pH 7.5, 0.2 M potassium sulfate, 20% PEG 3350, and 5% PEG 400. Streak seeding was necessary to obtain single crystals.
United States Latest News, United States Headlines
Similar News:You can also read news stories similar to this one that we have collected from other news sources.
SynDLP is a dynamin-like protein of Synechocystis sp. PCC 6803 with eukaryotic features - Nature CommunicationsIn eukaryotes, dynamins and dynamin-like proteins (DLPs) are involved in various membrane remodeling processes. Here, the authors present the structure and functional characterization of a DLP of the cyanobacterium Synechocystis sp. PCC 6803.
Read more »
The most at-risk regions in the world for high-impact heatwaves - Nature CommunicationsThe global risk of record-breaking heatwaves is assessed, with the most at-risk regions identified. It is shown that record-smashing events that currently appear implausible could happen anywhere as a result of climate change.
Read more »
Smart thrombosis inhibitors without bleeding side effects via charge tunable ligand design - Nature CommunicationsTreatments to prevent thrombosis are suboptimal. Here, the authors identify a lead an antithrombotic drug targeting polyphosphate based on switchable protonation states for the anion-binding groups, demonstrating antithrombotic activity in multiple mouse models, not causing bleeding, and well tolerated.
Read more »
More NYC parking garages have structural flaws, need immediate fix: official“We currently reviewed garage records and identified garages with Class 1 violations,” said acting Department of Buildings Commissioner Kazimir Vilenchik, adding that such infractions a…
Read more »
Trump loses appeal to block Pence from testifying about direct communications | CNN PoliticsFormer President Donald Trump has lost an emergency attempt to block former Vice President Mike Pence from testifying about their direct conversations, in the latest boost to a federal criminal investigation examining Trump's and others' actions after the 2020 election.
Read more »
Verizon’s stock heads for best day in two years amid sharp rally in communications stocksVerizon Communications Inc.’s stock was headed for its best day in more than two years Thursday amid a rally in the communications sector. Verizon’s stock...
Read more »