How a genetic mutation can cause individuals with normal cholesterol to develop heart disease at a young age UTHealth
mice were placed on HFD for 12 weeks starting at 8 weeks of age, at the end of the tamoxifen injection regimen. For pravastatin treatment, pravastatin was dissolved in drinking water at a final dose of 50 mg/kg body weight/day and the mice hadaccess to it. The drinking water containing the drug was replaced with a freshly prepared solution every 7 days.
Analysis of plaque burden was performed in a blinded fashion by two researchers using ImageJ software and expressed as percentage area covered in plaque relative to the area of the entire aorta.Aortic roots and ascending aortas from experimental mice were fixed in 10% formalin solution, embedded in paraffin and then cut into 5 μM sections.
Tissues were isolated from the aortic root to the distal end of the aortic arch from four age-matched male SMC lineage-traced
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