Single primer enrichment technology: A new genomic resource to investigate the diversity of lettuce germplasm

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Single primer enrichment technology: A new genomic resource to investigate the diversity of lettuce germplasm
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For the first time, the single primer enrichment technology (SPET)—a novel high-throughput genotyping method—was used in lettuce to study the genetic diversity of a collection of 160 Lactuca accessions originating from 10 countries in Europe, America and Asia, and to identify genomic regions that underpin important agronomic traits.

In recent years, technologies to investigate the genomic diversity of crops have made extraordinary progress and novel methodologies such as single primer enrichment technology offer promising and cost-effective opportunities. SPET has so far been used in several

The EVA initiative was established in 2019 by ECPGR to improve knowledge of crop genetic diversity and exploit it to breed more resilient crops that can meet the major problems facing agriculture. The studied genotypes encompassed cultivars, breeding materials, and landraces originating from ten different countries in Europe, America and Asia, included different horticultural types, such as Butterhead, Iceberg, Romaine, Batavia, Crisp, and were phenotyped in multilocation field trials across three countries.

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