A history of cellular events is recorded in self-assembling protein chains
, brain slices were blocked overnight at 4 °C in MAXBlock blocking medium, followed by four washes for 30 min each at RT in MAXWash washing medium. Next, slices were incubated with primary antibodies in MAXbind staining medium overnight at 4 °C, and then washed in MAXWash washing medium four times for 30 min each at RT.
Acryloyl-X amino)hexanoic acid, succinimidyl ester was resuspended in anhydrous dimethylsulfoxide at a concentration of 10 mg mland stored in a desiccated environment at −20 °C. For anchoring, cells were incubated in 200 μl AcX at a concentration of 0.1 mg mlin a 2-ethanesulfonic acid -based saline overnight at 4 °C. Then, cells were washed with 1× PBS three times at RT for 5 min each.′-methylenebisacrylamide , 1.5 ppt ammonium persulfate and 1.
For immunostaining and imaging, gels were first incubated in bovine serum albumin blocking solution that contains 1% BSA, 0.5% Triton X in 1× PBS for 1 h at RT then with primary antibodies in MAXbind staining medium overnight at 4 °C. Gels were washed three times in BSA blocking solution for 30 min each at RT and incubated with fluorescently labeled secondary antibodies in MAXbind Staining Medium overnight at 4 °C.
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