Phenotyping single-cell motility in microfluidic confinement

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Phenotyping single-cell motility in microfluidic confinement
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New technology maps movement of microscopic algae in unprecedented detail uniofexeter elife

. Your article has been reviewed by 2 peer reviewers, one of whom is a member of our Board of Reviewing Editors, and the evaluation has been overseen by Anna Akhmanova as the Senior Editor. The reviewers have opted to remain anonymous. We regret the lengthy delay in furnishing this decision letter.

2) Secondarily, the abstract and indeed a fair amount of the paper is not written in a form that will be easily understood by biologists with only limited background in dynamical systems ideas and the kind of technical jargon that has been used [e.g. words like 'microhabitat' and 'deterministic fusion].1) The main suggestion to the authors is to take at least some of their detailed observations and analyze them with appropriate theory to see if agreement can be found .

In the original submission, we had indicated that our experimental results did not agree with the conclusions of the Cammann et al. paper – regarding the appearance of steady-state flux loops inconfinement. Indeed, while such a swimmer should be expected to perform circular movement, it should do so with equal probability clockwise versus counter clockwise. Therefore, there should be no flux loops averaged over long-times.

We further ruled out any asymmetries in the microwells themselves by performing confocal imaging of trapped droplets, which confirmed that the droplets have a rounded spherical geometry, as illustrated in figure 1B.

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