Nature research paper: Origin and evolutionary malleability of T cell receptor α diversity
) and adult trout were obtained from fish dealers. The blood samples from three female adult African bush elephants were obtained from the Wuppertal Zoological Garden and provided by L. Grund. All animal experiments were performed in accordance with relevant guidelines and regulations, approved by the review committee of the Max Planck Institute of Immunobiology and Epigenetics and the Regierungspräsidium Freiburg, Germany .Animals were euthanized using 0.02% MESAB.
Mouse lymphocytes were obtained from either the thymus or the spleen ; cells were passed through a cell strainer in PBS, centrifuged and the cell pellet dissolved in TRIzol following the recommendations of the manufacturer. For elephant blood samples, mononuclear cells were isolated from roughly 50 ml of peripheral blood as described in ref.Percoll condition; cells were washed and resuspended in TRIzol. Total RNAs were extracted from TRIzol according to the manufacturer’s protocol..
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