A team of researchers has developed an innovative imaging platform that promises to improve our understanding of cellular structures at the nanoscale.
This platform, called soTILT3D for single-objective tilted light sheet with 3D point spread functions , offers significant advancements in super-resolution microscopy, enabling fast and precise 3D imaging of multiple cellular structures while the extracellular environment can be controlled and flexibly adjusted.
The soTILT3D platform directly addresses these challenges. By synergistically integrating an angled light sheet, a nanoprinted microfluidic system and advanced computational tools, soTILT3D significantly improves imaging precision and speed, allowing for clearer visualization of how different cellular structures interact at the nanoscale -- even in conventionally challenging samples.
"The design and geometry of the microfluidic chip and nanoprinted insert with the micromirror can be easily adapted for various samples and length scales, providing versatility in different experimental setups," said Nahima Saliba, co-first author of the paper alongside fellow graduate student Gabriella Gagliano, who is also associated with the Smalley-Curl Institute and the Applied Physics Graduate Program at Rice.
SoTILT3D's microfluidic device also supports automated Exchange-PAINT imaging, allowing different targets to be visualized sequentially without the color offsets common in multicolor approaches when imaging in-depth at the nanoscale.The soTILT3D platform has demonstrated remarkable improvements in imaging precision and speed.
"Our goal with soTILT3D was to create a flexible imaging tool that overcomes limitations of traditional super-resolution microscopy," said Gustavsson."We hope these advancements will enhance studies in biology, biophysics and biomedicine, where intricate interactions at the nanoscale are key to understanding cellular function in health and pathogenesis."
Optics Medical Technology Ultrasound Computational Biology Statistics Computer Graphics Mobile Computing
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