New method tracking changes in bloodvessels could advance brain disease detection BrownUniversity NatureComms
for potential impact of long-term craniotomy and imaging on animal physiology). The mice were housed in a controlled environment with a 12-h light/dark cycle, with lights on at 7:00 a.m. and off at 7:00 p.m. The ambient temperature was maintained at a constant 22 ± 2 °C, and the relative humidity was kept at 50 ± 10%.
The base of the window consisted of two 3-mm glass cover slips and the apex consisted of one 5-mm circular glass slip; these were epoxied together with a transparent UV-cured resin. The thickness of the 3 mm-wide base of the window was 0.24-mm , closely approximating the thickness of the mouse parietal bone .
The use of an open-skull cranial window has clear strengths and weaknesses against a thinned-skull cranial window. The open-skull method in this study provides higher quality microangiography where individual capillaries are clearly visualized (Fig.
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