Copper, but not silver, is effective against SARS-CoV-2 on surfaces ruhrunibochum SciReports
pieces , 25 µL of SARS-CoV-2 was spotted in the middle of each test sample and incubated for 1 h and 24 h at room temperature and 32 ± 1% humidity. Virus was recovered by adding 225 µL of Dulbecco’s modified Eagle’s medium fetal calf serum , 1% non-essential amino acids, 100 IU/mL penicillin, 100 µg/mL streptomycin and 2 mM L-Glutamine).
According to the antimicrobial tests silver acetate and copper sulfate solutions were used as ionic controls. Therefore, SARS-CoV-2 was spiked with different concentrations of AgAc and CuSOsolutions and was incubated for 1 h and 24 h at room temperature. Remaining viral titers were again quantified by an endpoint dilution assay followed by TCIDICP-MS was used to determine the amount of ions released into the medium containing the virus during contact with the surface of interest.
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