Science, Space and Technology News 2024
Researchers at MIT have developed a technique to map the interactions between genes and enhancers in cells, providing insights into genetic regulation. By observing the timing of enhancer and gene activation, this method aids in identifying potential drug targets for genetic disorders.researchers have innovated a method to observe the interaction between genes and enhancers by monitoring their activation times, helping to pinpoint drug targets for genetic disorders.
More recently, in 2010, researchers discovered that these enhancers are transcribed into RNA molecules, known as enhancer RNA or eRNA. Scientists suspect that this transcription occurs when the enhancers are actively interacting with their target genes. This raised the possibility that measuring eRNA transcription levels could help researchers determine when an enhancer is active, as well as which genes it’s targeting.
While brainstorming ideas for new ways to capture eRNA, Mahat and Sharp considered using click chemistry, a technique that can be used to join two molecules together if they are each tagged with “click handles” that can react together. “You want to be able to determine, in every cell, the activation of transcription from regulatory elements and from their corresponding gene. And this has to be done in a single cell because that’s where you can detect synchrony or asynchrony between regulatory elements and genes,” Mahat says.
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