Nature research paper: Increasing the resilience of plant immunity to a warming climate
. Peak selection and integration of acquired MRM data files was done using QuanLynx v4.1 software or Quantitative Analysis program in MassHunter software . Analyte levels were calculated as previously indicatedApproximately 0.1–0.2 g of ground plant tissues were dissolved in nuclei isolation buffer ) on ice or at 23 °C or 28 °C .
After 30 min incubation on ice, 625 µl of ChIP dilution buffer were added and the samples were sonicated for 1 min in the cold room using Sonic Dismembrator or 5–6 min using Bioruptor . After adding 200 µl of ChIP dilution buffer and 100 µl of 10% Triton X-100, samples were spun at full speed for 5 min to remove debris. For pre-clearing, samples were incubated with 25 µl of magnetic protein A or G beads for 2 h in the cold room.
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