Gigapixel 3D microscope captures life in unprecedented detail NaturePhotonics
By the following day, the duo sorted out their software issues and demonstrated a successful proof-of-principle device on the classic children's puzzle book. By combining 24into a single platform and stitching their images together, they created a single camera capable of taking gigapixel images over an area about the size of a piece of paper.
The latest version of MCAM relies on 54 lenses with higher speed and resolution than the prototype that found Waldo. Building upon recent work completed in close collaboration with Dr. Eva Naumann's lab at Duke, innovative software gives the microscope the ability to take 3D measurements, provide more detail at smaller scales and make smoother movies.
In the paper, McCarroll and his group describe interesting movements they'd never seen before thanks to using this camera. The 3D capabilities of the MCAM, coupled with its all-encompassing view, allowed them to record differences in the fish's pitch, whether they trended toward the top or bottom of their tanks and how they tracked prey.
In previous studies, the researchers needed to anesthetize and mount the developing fish to keep them steady while measurements were taken with lasers. But knocking them out for prolonged periods of time might also cause changes in their development that could skew the experiment's results. With the help of the new MCAM, the researchers have shown that they're able to get all of these measurements while the fish live their lives unencumbered, no knockouts or clamps required.
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