Frontiers | Co-sensitization between legumes is frequently seen, but variable and not always clinically relevant

Common meat-free proteins may trigger soybean and peanut allergies in some people frontiersin

). In short, the globulin fraction was collected by solubilizing defatted legume meal with a high salt buffer and centrifuging. The supernatant was collected afterwards which contained the globulins that dissolved in the high salt buffer. By using 100 kDa ultra-filtration, the supernatant was divided in a 7S and 11S globulin fraction and the 2S albumin fraction.

Four microliters of the protein fractions were loaded at a 500 nl/min flow rate onto the analytical column, and peptides were eluted through the reversed-phase columna 45-minute linear gradient. The MS data were processed using Proteome Discoverer v.1.4 and SEQUEST as search engine. Proteins were identified by searching the mass spectrometric data against the Uniprot Fabaceae database accessed on September 2017. Protein hits based on 2 successful peptide identifications were considered valid.

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