Understanding the differences between healthy and type 2 diabetes-affected pancreaticislets UofIllinois
-Asp separations were prepared as follows. A 300 mM 2-ethanesulfonic acid was prepared in water and pH adjusted with 5 M NaOH. Additionally, a 200 mM potassium bromide in water, a 10% quaternary ammonium β-cyclodextrin solution in water, and a stock of 1 M citric acid in water were prepared. All mentioned stock solutions were stored at 4 °C.
For Zipchip-CE-ESI-MS analysis, the dried samples after desalting were reconstituted in acidified Metabolites Diluent containing 1 μM quinine as an internal control. Stock standards ranging from 0.1–150 μM for GABA, 0.1–10 μM for glutamate, synephrine, norepinephrine, and-DOPA, and 0.1–5 μM for serotonin, acetylcholine, dopamine, tyramine, epinephrine, and tryptamine were prepared in water with 1% formic acid.
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