New 'CRISPR-Combo' method boosts genome editing power in plants UofMaryland NaturePlants
weeks. The calli of both vectors were grown on regeneration I medium for seven weeks by subculturing every two weeks.target site. A total of 10 and 16 individual transgenic plants were examined for Cas9-Act3.0-based GE and A+GE vectors, respectively. GE, genome editing. A+GE, simultaneous gene activation and editing., Representative images of Cas9-Act3.0-induced rice plants from hormone-containing and hormone-free mediums.
in a HF manner. RSM, rice selection medium. All calli were subcultured with HF fresh medium every two weeks., Efficiency of Cas9-Act3.0-callus induction and regeneration in a HF manner. All calli were cultured under the same conditions., Number of regenerated transgenic plants per Cas9-Act3.0-mediated callus in a HF manner. Each dot indicates the seedling number generated from one transgenic callus. Error bar represents the mean ± s.d. .mutants in a HF manner.
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