Synthetic biology meets fashion in engineered silk wustl NatureComms
. Spinning dopes were prepared by the dissolution of lyophilized protein powders in HFIP. The resulting solution was loaded to a 100 μL Hamilton syringe , and extruded into a 95% v/v methanol bath using a Harvard Apparatus Pump 11 Elite syringe pump at a controlled protrusion rate of 10 μL/min. To achieve pH-controlled spinning, either 5 mM CHwas added to the 95% v/v methanol bath to adjust the pH to 5.5 or 11, respectively.
Specifically, the ultimate tensile strength was calculated as the maximum load divided by the initial fiber cross-sectional area, which was determined by light microscopy before testing, assuming a circular cross-section. The modulus was obtained by fitting a linear least-square regression line to the initial elastic region of the stress-strain curve. The ultimate breaking strain was calculated as the percentage of fiber elongation relative to the initial gauge length of 5 mm before fracture.
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