Nature research paper: Androgens increase excitatory neurogenic potential in human brain organoids
) Droplet digital PCR results showing the decrease in AR transcription between days 17 and 25. ref=housekeeping gene EIF2B2.) Representative images of the cortical wall at 21, 35 and 52d, with DAPI-labelled nuclei visible, showing the relative reduction in the radial glial progenitor layer and an increase in the thickness of the neuronal layer over time.) Quantification of the percentage of cells , containing AR mRNA puncta , out of all cells at 21, 35 and 52d.
control; EGFP-C1-AR-V7: constitutively active AR. Immunostaining for GFP and TBR2. Yellow dashed lines demarcate the apical and basal boundaries of the VZ. Note increased GFP+ nuclei in the VZ in EGFP-C1-AR-V7.) Immunostaining for GFP and Ki67 on XX 50d organoids, electroporated at 45d. Co-stained with DAPI .
control plasmid; EGFP-C1-AR-V7: plasmid expressing constitutively active AR; EGFP-C1-ERaY537S: plasmid expressing constitutively active ERa.) Quantification of the proportion of GFP+ cells co-staining for the proliferation marker Ki67 at 5 days post electroporation in XX organoids electroporated with the indicated plasmid at 45d.
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