This article explores the effectiveness of the Absorbance 96 plate reader in measuring optical density (OD600) for bacterial growth assessments, in comparison with traditional spectrophotometry methods.
Sponsored Content by ByonoyApr 19 2024Reviewed by Maria Osipova The OD values acquired by Absorbance 96 were comparable to those derived from a different commercial plate reader. The data from Absorbance 96 confirmed its strength and dependability in obtaining OD600 measurements for bacterial growth assays. The Absorbance 96 's compact design allows for the performance of flexible OD measurements and improves laboratory productivity and effectiveness.
This article details the Absorbance 96 plate reader’s ability to determine OD600 measurements. Working with HM Munich University of Applied Sciences, a comparative analysis of Absorbance 96 using a different spectrophotometer and microplate reader was conducted. The purpose was to observe the bacterial growth of a ureolytic bacterium Sporosarcina pasteurii, known for ts unique ability to microbially induce calcite precipitation necessary for constructional engineering and material applications.
Triplicate OD calculations of the dilution series of Sporosarcina pasteurii in a 96-well microplate were completed at 605 nm. The SpectraMax iD3 was first, then the same microplate was measured with the Absorbance 96. The achieved OD605 results from the two devices were comparable with insignificant differences in measurements ).
The variance in the absolute value of OD605 measured by the photometer is impacted by how the light beam passes through the specimen cuvettes horizontally in photometers with the path length of a standardized 1 cm. When assessing the workflow for OD600 measurements, Absorbance 96 has a footprint advantage over other large traditional plate readers. Its compact size improves moveability, such as making on-site OD600 measurement at the workstation possible without requiring specimen transport to specific locations housing plate readers.
Bacterial Growth Analysis Optical Density OD600
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