MHZ9 modulates ethylene signaling at translational level in rice NatureComms
transgenic plants treated with 10 μL/L of ethylene and were UV-crosslinked with 600 mJ/cmat 254 nm for three times, using a UVP CX-2000 Shortwave Ultraviolet Crosslinker . Add 1 mL of TRIzol per 100 mg powdered tissue sample and lyse the samples by votexing and incubating for 5 min at room temperature . Add 0.2 mL of chloroform per 1 mL of TRIzol reagent used for lysis to induce phase separation.
transgenic rice seedlings after treatment with 10 μL/L of ethylene for 8 h were used . The wild-type rice seedlings under the same conditions were used as negative control . After immunoprecipitation with GFP-Trap_A beads, the immunoprecipitated proteins were eluted by 50 mM glycine and neutralized with 1 M Tris buffer .
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