A new E.coli strain is designed with an amino-acid swapped genetic code, making it resistant to viruses and also unable to replicate outside of a lab setting, preventing unwanted gene flow. NBThighlight
). Early exponential phase cultures of MDS42 and Syn61∆3 were infected at an MOI of 0.001 with the corresponding phages, and free phage titers were determined after 24 h of incubation. Measurements were performed in n = 3 independent experiments or in n = 2 independent experiments ; dashed line represents input phage titer without bacterial cells ; dots represent data from independent experiments; bar graphs represent the mean; error bars represent the SEM based on n = 3 independent experiments.
The time-course kinetics of host and viral tRNA expression in Syn61∆3 cells following REP12 phage infection was quantified using tRNAseq . The endogenoustRNAs of the host Syn61∆3 are highlighted in green and blue, respectively, while the tRNA-Serof the REP12 virus is highlighted in red. REP12 viral tRNAs are shown in light red; endogenous tRNAs of Syn61∆3 are shown in gray. Data represent mean TPM . Source data is available within this paper.
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